AKT1 Expression Vectors, Lentivirus & CRISPR Knockout Vectors
Enhance your research with ready-to-use AKT1-specific vectors and viral particles designed for reliable AKT1 overexpression, knockout, and silencing across a wide range of experimental systems. abm offers a complete portfolio of validated molecular tools – including AKT1 CRISPR knockout vectors, lentiviral particles, AAV vectors, adenovirus, ORF clones, and siRNA constructs for both in vitro and in vivo applications.
Human AKT1 Gene Overview
| Gene Name | AKT serine/threonine kinase 1 |
|---|---|
| Gene Symbol | AKT1 |
| Synonyms | AKT,PKB,RAC,PRKBA,PKB-ALPHA,RAC-ALPHA |
| NCBI ID | 207 |
| Ensembl ID | ENSG00000142208 |
| Uniprot ID | P31749 |
| Gene Type | Protein coding |
Gene Summary
This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020]
Human AKT1 Related Products
| Product Type | Key Applications | Cat. No. | Product |
|---|---|---|---|
| Lentiviral Vector & Virus | Stable overexpression | LC111710 | View Product |
| AAV Vector & Virus | Long-term expression | VC111710 | View Product |
| Adenovirus | Transient expression | A211710 | View Product |
| CRISPR KO Tools | Gene knockout (Lentiviral) | LG111710 | View Product |
| Gene knockout (non-viral) | PG111710 | View Product | |
| Gene knockout (AAV) | VG111710 | View Product | |
| CRISPR Activation | Gene activation | LA111710 | View Product |
| siRNA Tools | Gene knockdown (Lentiviral) | LS111710 | View Product |
| Gene knockdown (AAV) | VS111710 | View Product | |
| ORF Vector | Template DNA | PC111710 | View Product |
Mouse Akt1 Gene Overview
| Gene Name | Akt serine/threonine kinase 1 |
|---|---|
| Gene Symbol | Akt1 |
| Synonyms | Akt,PKB,Rac,LTR-akt,PKB/Akt,PKBalpha |
| NCBI ID | 11651 |
| Ensembl ID | ENSMUSG00000001729 |
| Uniprot ID | P31750 |
| Gene Type | Protein coding |
Gene Summary
This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Mouse Akt1 Related Products
| Product Type | Key Applications | Cat. No. | Product |
|---|---|---|---|
| Lentiviral Vector & Virus | Stable overexpression | LC511710 | View Product |
| AAV Vector & Virus | Long-term expression | VC411710 | View Product |
| Long-term expression | VC511710 | View Product | |
| Adenovirus | Transient expression | A511710 | View Product |
| CRISPR KO Tools | Gene knockout (Lentiviral) | LG411710 | View Product |
| Gene knockout (non-viral) | PG411710 | View Product | |
| Gene knockout (AAV) | VG411710 | View Product | |
| CRISPR Activation | Gene activation | LA411710 | View Product |
| siRNA Tools | Gene knockdown (Lentiviral) | LS411710 | View Product |
| Gene knockdown (AAV) | VS411710 | View Product | |
| ORF Vector | Template DNA | PC411710 | View Product |
| Template DNA | PC511710 | View Product |
Rat Akt1 Gene Overview
| Gene Name | AKT serine/threonine kinase 1 |
|---|---|
| Gene Symbol | Akt1 |
| Synonyms | Akt |
| NCBI ID | 24185 |
| Ensembl ID | ENSRNOG00000028629 |
| Uniprot ID | P47196 |
| Gene Type | Protein coding |
Gene Summary
Enables several functions, including ATP binding activity; GTPase activating protein binding activity; and enzyme binding activity. Involved in several processes, including cellular hyperosmotic salinity response; protein phosphorylation; and regulation of protein metabolic process. Acts upstream of or within several processes, including negative regulation of cell size; positive regulation of sodium ion transport; and regulation of protein localization. Located in cytosol; dendrite; and nucleus. Used to study artery disease (multiple); autosomal recessive polycystic kidney disease; heroin dependence; impotence; and type 2 diabetes mellitus. Biomarker of several diseases, including brain ischemia; liver disease (multiple); opiate dependence; type 2 diabetes mellitus; and ureteral obstruction. Human ortholog(s) of this gene implicated in several diseases, including Cowden syndrome 6; breast cancer (multiple); gastrointestinal system cancer (multiple); pancreatic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human AKT1 (AKT serine/threonine kinase 1). [provided by Alliance of Genome Resources, Apr 2025]
Rat Akt1 Related Products
| Product Type | Key Applications | Cat. No. | Product |
|---|---|---|---|
| Lentiviral Vector & Virus | Stable overexpression | LC611710 | View Product |
| AAV Vector & Virus | Long-term expression | VC611710 | View Product |
| Adenovirus | Transient expression | A611710 | View Product |
| CRISPR KO Tools | Gene knockout (Lentiviral) | LG611710 | View Product |
| Gene knockout (non-viral) | PG611710 | View Product | |
| Gene knockout (AAV) | VG611710 | View Product | |
| CRISPR Activation | Gene activation | LA611710 | View Product |
| siRNA Tools | Gene knockdown (Lentiviral) | LS611710 | View Product |
| Gene knockdown (AAV) | VS611710 | View Product | |
| ORF Vector | Template DNA | PC611710 | View Product |
Accelerate Discovery with Research-Ready Constructs
| Why Researchers Choose abm | Workflow Benefits |
|---|---|
| Gene-Specific Constructs | Eliminate time-consuming cloning and accelerate project timelines |
| Available as Plasmid DNA or Packaged Virus | Choose delivery format that best fits your experiment |
| Validated Sequence Design | Ensure reliable, reproducible experimental performance |
| Available in Multiple Species Options | Simplify cross-species and translational research studies |
| Flexible Customization Services | Tailor constructs with different promoters, tags, markers and other modifications |
| Expert Technical Support | Get guidance from gene delivery specialists |
Product Overview & Key Features
| System | Applications | Key Features |
|---|---|---|
| CRISPR Knockout Vectors & Viruses | Targeted gene disruption & functional knockout studies | •Pre-designed validated sgRNAs •Available in All-in-One Cas9 + sgRNA or sgRNA-only formats •Available as lentivirus, AAV and non-viral •Ideal for stable genome editing |
| Lentiviral Expression Vectors & Particles | Stable gene overexpression or delivery into difficult-to-transfect cells | •Long-term expression through genomic integration •Highly efficient in dividing and non-dividing cells •Ideal for stable cell line generation |
| AAV Vectors & Packaged Virus | In vivo delivery & transient expression with low immunogenicity | •Multiple serotypes available for tissue targeting •Excellent for animal studies and sensitive primary cells •Episomal expression with strong safety profile |
| Adenovirus | High-efficiency transient expression | •Rapid, robust gene expression •Large cargo capacity •Ideal for primary cells and strong short-term expression studies |
| ORF Vector | Template for coding sequence | •Ready-to-use full-length coding sequences •Low cost and convenient alternative to gene synthesis |
| siRNA Knockdown Tools | Temporary gene silencing & pathway validation | •Robust gene knockdown •Ideal for target validation and preliminary functional screening •Available as Lentivirus, AAV or synthetic dsRNA oligos |