FNDC3CP ORF Vector (Human)
LINC00523 CRISPR
LINC00523 Lentiviral
LINC00523 AAV
LINC00523 Adenovirus
LINC00523 ORF
LINC00523 siRNA
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Retired Cat.No.
20722011 -
Product Name
FNDC3CP ORF Vector (Human) -
Unit
1.0 µg DNA -
Description
The FNDC3CP ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning. -
Gene Name/Gene ID
LINC00523, 283601 -
Accession Number
-
Species
Human -
System
Non-Viral Vector -
Insert Size
1627.0 -
Vector Size
2596 -
Storage Condition
Store ORF Vectors at -20°C. -
Disclaimer
1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final. 2. Disclaimer for gene sequence: The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final. 3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s). 4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene. 5. Disclaimer for the stop codon: The stop codon for the inserted gene is not included in our ORF vectors. Our ORF vectors contain open ORF inserts (no stop codon) to allow for subsequent insertion into other vectors with C-terminal tags or C-terminal fusion proteins if desired. Please note that a stop codon will need to be added by PCR to the end of the gene insert if no tag or fusion protein is needed in subsequent vectors. -
Guarantee
abm guarantees that the correct ORF construct is provided. If this is not the case, we will provide a one-time replacement. The replacement will not be covered by the same guarantee. -
Caution
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Print/Download Datasheet
Publishing research using PC120722? Please let us know so that we can cite the reference in this datasheet.
PC120722 has not been cited in any literature.
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Verify plasmid quality: Confirm the plasmid is intact, at sufficient concentration, and free of contaminants (e.g., salts, ethanol, nucleases).
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Check the host strain: Use a suitable cloning strain (e.g., recA⁻/endA⁻ such as DH5α) and confirm cells are competent and not expired.
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Confirm selection conditions: Make sure the correct antibiotic is used at the proper concentration and that plates/media are fresh.
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Review transformation conditions: Ensure the transformation method (chemical or electrocompetent) and recovery time are appropriate.
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Assess plasmid compatibility: Large, low-copy, toxic, or unstable plasmids may require specialized strains or growth conditions.
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Inspect growth conditions: Verify incubation temperature, shaking speed, and culture time.
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Check plasmid sequence/features: Look for toxic genes, strong promoters, or repeats that may reduce stability.
ABM community
Verified customer
Asked on Jan 30 2026
Answer
Standard delivery of abm’s vectors are in liquid format supplied in TE buffer (10mM Tris, 1mM EDTA, pH 8.0). Our vectors can also be ordered and delivered in agar stabs for an additional fee.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 30 2026
Answer
Typically vectors are supplied at 100ng/µl.
ABM Scientific Support
Answered on Jan 30 2026
Question
ABM community
Verified customer
Asked on Jan 30 2026
Answer
We recommend storing abm’s vectors at -20°C and avoiding repeated freeze/thaws as this may affect plasmid integrity and cloning efficiency.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 30 2026
Answer
A general Plasmid Amplification Protocol can be found here.
We recommend transforming abm’s plasmids into our ProClone™ Competent Cells (Cat. No. E003). The ProClone™ Competent Cells are high-efficiency chemically competent DH5α (E. coli) cells ideal for routine plasmid amplification due to its high yield, rapid growth, and high transformation efficiency, with recA⁻ and endA⁻ mutations that ensure plasmid stability and high DNA quality. Other common compatible cloning strains include TOP10 (DH10B derivatives).
We recommend transforming abm’s plasmids into our ProClone™ Competent Cells (Cat. No. E003). The ProClone™ Competent Cells are high-efficiency chemically competent DH5α (E. coli) cells ideal for routine plasmid amplification due to its high yield, rapid growth, and high transformation efficiency, with recA⁻ and endA⁻ mutations that ensure plasmid stability and high DNA quality. Other common compatible cloning strains include TOP10 (DH10B derivatives).
ABM Scientific Support
Answered on Jan 30 2026
Question
ABM community
Verified customer
Asked on Jan 30 2026
Answer
1. Use a sterile loop or pipette tip to scrape a small amount of cells from the agar stab. Only a tiny amount is needed as it contains live E. coli.
2. Gently streak the cells onto an agar plate containing the correct antibiotic selection in order to achieve single isolated colonies.
3. Place the plate “agar side up” and incubate at 37°C overnight.
4. Select a single colony from the plate for downstream applications, such as an overnight broth culture and grow E. coli to late exponential-early stationary phase. 4a. The broth culture can be subjected to miniprep plasmid extraction. We recommend using abm’s Column-Pure Plasmid Miniprep Kit (Cat. No. G4003). 4b. The broth culture can be used to prepare a glycerol stock for long-term storage. Mix culture with sterile glycerol to a final concentration of 15% and store at -80°C.
2. Gently streak the cells onto an agar plate containing the correct antibiotic selection in order to achieve single isolated colonies.
3. Place the plate “agar side up” and incubate at 37°C overnight.
4. Select a single colony from the plate for downstream applications, such as an overnight broth culture and grow E. coli to late exponential-early stationary phase. 4a. The broth culture can be subjected to miniprep plasmid extraction. We recommend using abm’s Column-Pure Plasmid Miniprep Kit (Cat. No. G4003). 4b. The broth culture can be used to prepare a glycerol stock for long-term storage. Mix culture with sterile glycerol to a final concentration of 15% and store at -80°C.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 29 2026
Answer
ABM Scientific Support
Answered on Jan 29 2026
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Current vector selected:
FNDC3CP ORF Vector (Human)
Cat. No.
PC120722
Plasmid DNA Services
Midiprep Plasmid Amplification (Add-on)
10-20 µg
$75.00
Maxiprep Plasmid Amplification (Add-on)
80-100 µg
$150.00
Bacterial Agar Stab (Add-on)
1 stab
$40.00
Controls and Related Products
Column-Pure Gel and PCR Clean-Up Kit
D516
100 Preparations
$180.00
Pro Ligation-Free Cloning MasterMix
G4004
50 Reactions
$258.00
MegaFi™ Pro Fidelity 2X PCR MasterMix
G887
800 rxn (10.0 ml)
$335.00
SafeView™ Classic
G108
1.0 ml
$78.00
Empty
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Current vector selected:
FNDC3CP ORF Vector (Human)
Cat. No.
PC120722
Controls and Related Products
Column-Pure Gel and PCR Clean-Up Kit
D516
100 Preparations
$180.00
Pro Ligation-Free Cloning MasterMix
G4004
50 Reactions
$258.00
MegaFi™ Pro Fidelity 2X PCR MasterMix
G887
800 rxn (10.0 ml)
$335.00
SafeView™ Classic
G108
1.0 ml
$78.00
Empty
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