WT1 Expression Vectors, Lentivirus & CRISPR Knockout Vectors
Enhance your research with ready-to-use WT1-specific vectors and viral particles designed for reliable WT1 overexpression, knockout, and silencing across a wide range of experimental systems. abm offers a complete portfolio of validated molecular tools – including WT1 CRISPR knockout vectors, lentiviral particles, AAV vectors, adenovirus, ORF clones, and siRNA constructs for both in vitro and in vivo applications.
Human WT1 Gene Overview
| Gene Name | WT1 transcription factor |
|---|---|
| Gene Symbol | WT1 |
| Synonyms | GUD,AWT1,WAGR,WT-1,WT33,NPHS4,WIT-2 |
| NCBI ID | 7490 |
| Ensembl ID | ENSG00000184937 |
| Uniprot ID | P19544 |
| Gene Type | Protein coding |
Gene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
Human WT1 Related Products
| Product Type | Key Applications | Cat. No. | Product |
|---|---|---|---|
| Lentiviral Vector & Virus | Stable overexpression | LC150474 | View Product |
| AAV Vector & Virus | Long-term expression | VC150474 | View Product |
| Adenovirus | Transient expression | A250474 | View Product |
| CRISPR KO Tools | Gene knockout (Lentiviral) | LG150474 | View Product |
| Gene knockout (non-viral) | PG150474 | View Product | |
| Gene knockout (AAV) | VG150474 | View Product | |
| CRISPR Activation | Gene activation | LA150474 | View Product |
| siRNA Tools | Gene knockdown (Lentiviral) | LS150474 | View Product |
| Gene knockdown (AAV) | VS150474 | View Product | |
| ORF Vector | Template DNA | PC150474 | View Product |
Mouse Wt1 Gene Overview
| Gene Name | WT1 transcription factor |
|---|---|
| Gene Symbol | Wt1 |
| Synonyms | Wt-1,D630046I19Rik |
| NCBI ID | 22431 |
| Ensembl ID | ENSMUSG00000016458 |
| Uniprot ID | P22561 |
| Gene Type | Protein coding |
Gene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms. [provided by RefSeq, Apr 2013]
Mouse Wt1 Related Products
| Product Type | Key Applications | Cat. No. | Product |
|---|---|---|---|
| Lentiviral Vector & Virus | Stable overexpression | LC450474 | View Product |
| AAV Vector & Virus | Long-term expression | VC450474 | View Product |
| Adenovirus | Transient expression | A450474 | View Product |
| CRISPR KO Tools | Gene knockout (Lentiviral) | LG450474 | View Product |
| Gene knockout (non-viral) | PG450474 | View Product | |
| Gene knockout (AAV) | VG450474 | View Product | |
| CRISPR Activation | Gene activation | LA450474 | View Product |
| siRNA Tools | Gene knockdown (Lentiviral) | LS450474 | View Product |
| Gene knockdown (AAV) | VS450474 | View Product | |
| ORF Vector | Template DNA | PC450474 | View Product |
Rat Wt1 Gene Overview
| Gene Name | WT1 transcription factor |
|---|---|
| Gene Symbol | Wt1 |
| Synonyms | |
| NCBI ID | 24883 |
| Ensembl ID | ENSRNOG00000013074 |
| Uniprot ID | P49952 |
| Gene Type | Protein coding |
Gene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the human gene is mutated in a small subset of patients with Wilm's tumors. Authors of PMID:7926762 provide evidence that Wt1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Jul 2008]
Rat Wt1 Related Products
| Product Type | Key Applications | Cat. No. | Product |
|---|---|---|---|
| Lentiviral Vector & Virus | Stable overexpression | LC650474 | View Product |
| AAV Vector & Virus | Long-term expression | VC650474 | View Product |
| Adenovirus | Transient expression | A650474 | View Product |
| CRISPR KO Tools | Gene knockout (Lentiviral) | LG650474 | View Product |
| Gene knockout (non-viral) | PG650474 | View Product | |
| Gene knockout (AAV) | VG650474 | View Product | |
| CRISPR Activation | Gene activation | LA650474 | View Product |
| siRNA Tools | Gene knockdown (Lentiviral) | LS650474 | View Product |
| Gene knockdown (AAV) | VS650474 | View Product | |
| ORF Vector | Template DNA | PC650474 | View Product |
Accelerate Discovery with Research-Ready Constructs
| Why Researchers Choose abm | Workflow Benefits |
|---|---|
| Gene-Specific Constructs | Eliminate time-consuming cloning and accelerate project timelines |
| Available as Plasmid DNA or Packaged Virus | Choose delivery format that best fits your experiment |
| Validated Sequence Design | Ensure reliable, reproducible experimental performance |
| Available in Multiple Species Options | Simplify cross-species and translational research studies |
| Flexible Customization Services | Tailor constructs with different promoters, tags, markers and other modifications |
| Expert Technical Support | Get guidance from gene delivery specialists |
Product Overview & Key Features
| System | Applications | Key Features |
|---|---|---|
| CRISPR Knockout Vectors & Viruses | Targeted gene disruption & functional knockout studies | •Pre-designed validated sgRNAs •Available in All-in-One Cas9 + sgRNA or sgRNA-only formats •Available as lentivirus, AAV and non-viral •Ideal for stable genome editing |
| Lentiviral Expression Vectors & Particles | Stable gene overexpression or delivery into difficult-to-transfect cells | •Long-term expression through genomic integration •Highly efficient in dividing and non-dividing cells •Ideal for stable cell line generation |
| AAV Vectors & Packaged Virus | In vivo delivery & transient expression with low immunogenicity | •Multiple serotypes available for tissue targeting •Excellent for animal studies and sensitive primary cells •Episomal expression with strong safety profile |
| Adenovirus | High-efficiency transient expression | •Rapid, robust gene expression •Large cargo capacity •Ideal for primary cells and strong short-term expression studies |
| ORF Vector | Template for coding sequence | •Ready-to-use full-length coding sequences •Low cost and convenient alternative to gene synthesis |
| siRNA Knockdown Tools | Temporary gene silencing & pathway validation | •Robust gene knockdown •Ideal for target validation and preliminary functional screening •Available as Lentivirus, AAV or synthetic dsRNA oligos |