ZNF385D-AS2 Adenovirus (Human)
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Retired Cat.No.
51491051 -
Product Name
ZNF385D-AS2 Adenovirus (Human) -
Unit
1.0 ml -
Description
This adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express your gene of interest in a wide range of host cells. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells.
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Gene Name
ZNF385D-AS2 -
Accession Number
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Species
Human -
Titer
>1x106pfu/mL -
System
Adenovirus -
Promoter
CMV -
Insert Size
421.0 -
Vector Size
32908 -
Storage Condition
Storage Buffer: DMEM with 10% glycerol.
Upon arrival, store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
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Disclaimer
1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final. 2. The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final. 3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s). 4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene.
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Guarantee
abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement will not be covered by the same guarantee. Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.
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Caution
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
5’ YPYDVPDYA 3’
2. Virus preparation might be contaminated with bacteria. Use a 0.25µm syringe filter to clear the virus preparation and repeat transduction following strict sterile technique.
3. Cell line might be contaminated with mycoplasm. This type of contamination is often not immediately obvious. The contamination effect is amplified after virus transduction. Repeat transduction with fresh cells.
4. The virus preparation may contain some cell debris which could be mistaken as a change in cell morphology. Normally this issue will disappear 3-5 days after virus transduction.
1. qPCR detection is much more sensitive than Western blot, and any target signal is amplified greatly during qPCR.
2. Verify that your primary antibody is of good quality and include good positive and negative controls in your Western blot.
3. Western blot can only be performed after a stable cell line has been established and notably it rarely works well with a polyclonal cell culture.
4. mRNA expression does not always correlate with protein expression because many different biological factors may affect translation, including protein half-life, protein degradation and molecular processes such as phosphorylation, ubiquitination, methylation, etc. abm is not in a position to guarantee GOI expression at the protein level due to the number of experimental variables involved. abm guarantees GOI expression at the mRNA level only, while expression at the protein level must be determined experimentally. We recommend checking the literature to see whether other scientists have been able to over-express the protein in the same target cells and how this was achieved (tag, delivery system, detection method, etc).