Anti-CD44 Antibody
Cat. No.
Y059133
Unit
100 μg
Price
$442.00
| Cat. No. | Y059133 |
| Name | Anti-CD44 Antibody |
| Clone | Monoclonal |
| Category | Monoclonal Antibodies |
| Gene Name | CD44 |
| Alias | AU023126, AW121933, AW146109, HERMES,, IN, MC56, Pgp1, CD44R, HC |
| Application | WB,IP,Flow cyt |
| Application Info | Western Blotting recommended dilution: 1:500 Positive control: Kg-1a human acute leukemia cell lysate JURKAT human leukemia T-cell lysate Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. 60 min on vertical incubator Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). |
| Raised In | Mouse |
| Clonality | Monoclonal |
| Reactivity | Human,Other |
| Description | CD44 is a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. |
| Immunogen | COS-7 cells (African Green Monkey). |
| Isotype | IgG1 |
| Concentration | 1ug/ul |
| Recommended Dilutions | Western Blotting recommended dilution: 1:500 Positive control: Kg-1a human acute leukemia cell lysate JURKAT human leukemia T-cell lysate Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. 60 min on vertical incubator Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Human,Other |
| Specificity | The antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1) |
| Purity | Affinity purified |
| Guarantee | abm guarantees that all our Anti-CD44 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y059133 |
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