Recombinant Human IL4 (E. coli)

Cat. No.

Z100545

Unit

Price

$65.00

Cat. No.	Z100545
Name	Recombinant Human IL4 (E. coli)
Description	IL4 is produced mainly by a sub-population of activated T helper cells which also secrete IL5 and IL6. The biological activities of IL4 are mediated by a specific receptor, the extracellular domain of which is related to the receptors for EPO, IL6, and the beta chain of the IL-2 receptor. IL4 participates in several B-cell activation processes as well as of other cell types. A co-stimulator of DNA-synthesis, IL4 induces the expression of class II MHC molecules on resting B-cells, enhances both secretion and cell surface expression of IgE and IgG1, and also regulates the expression of the low affinity Fc receptor for IgE on both lymphocytes and monocytes. Pre-treatment of macrophages with IL4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma.
Organism	Human (H. sapiens)
Unit quantity	10 µg
Family	Other Interleukins
Tag	Tag Free
Aliases	BCGF-1, BCGF1, BSF-1, BSF1, IL-4
Gene Symbol	IL4
Gene ID	3565
Accession Number	P05112
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	15 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in sodium phosphate buffer with NaCl (pH 7.8)
Function	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100545

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.