Recombinant Human IL7 (E. coli)

Cat. No.

Z100979

Unit

Price

$7,520.00

Cat. No.	Z100979
Name	Recombinant Human IL7 (E. coli)
Description	IL7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL7.Human (152 amino acids; 17.4 kDa) and murine IL7 (129 amino acids) show 60 % sequence homology at the protein level. The human IL7 receptor is an integral strongly glycosylated membrane proteins of 76 kDa expressed on activated T-cells. This receptor has been designated as CD127. IL7 receptors are expressed on pre-B-cells and their progenitors. They are not expressed on mature B-cells. IL7 receptors are expressed also on bone marrow macrophages. Functional IL7 receptors are found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B- cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line
Organism	Human (H. sapiens)
Storage Condition	Ice Pack
Unit quantity	1.0 mg
Family	Other Interleukins
Tag	Tag Free
Aliases	IL-7, IMD130
Gene Symbol	IL7
Gene ID	3574
Accession Number	P13232
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	17.7 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in sodium phosphate and NaCl
Function	The ED50 as determined by dose-dependent stimulation of mouse lymphoma was <0.5ng/ml
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100979

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.