Recombinant Human RANTES (CCL5) (E. coli)

Cat. No.

Z100789

Unit

Price

$5,400.00

Cat. No.	Z100789
Name	Recombinant Human RANTES (CCL5) (E. coli)
Description	RANTES belongs to the family of chemotactic cytokines known as chemokines. RANTES is expressed by an early response gene. The synthesis of RANTES is induced by TNF-alpha and IL-1-alpha. RANTES is chemotactic for T cells, human eosinophils and basophils and plays an active role in recruiting leukocytes into inflammatory sites. RANTES also activates eosinophils to release, for example, eosinophilic cationic protein. It changes the density of eosinophils and makes them hypodense, which is thought to represent a state of generalized cell activation and is associated most often with diseases such as asthma and allergic rhinitis. RANTES also is a potent activator of oxidative metabolism specific for eosinophils. RANTES increases the adherence of monocytes to endothelial cells. It selectively supports the migration of monocytes and T lymphocytes expressing the cell surface markers CD4 and UCHL1. These cells are thought to be pre-stimulated T helper cells with memory T cell functions. RANTES activates human basophils from some select basophil donors and causes the release of histamines.
Organism	Human (H. sapiens)
Storage Condition	Ice Pack
Unit quantity	1.0 mg
Family	Chemokine-beta Family (CC)
Tag	Tag Free
Aliases	D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP
Gene Symbol	CCL5
Gene ID	6352
Accession Number	P13501
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	8 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2µm filtered solution in 50mM NaAc, pH 5.0, 100mM NaCl.
Function	Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100789

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Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.