Recombinant Human SCF (E. coli)
| Cat. No. | Z100819 |
| Name | Recombinant Human SCF (E. coli) |
| Description |
SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL-7, Epo and some other growth and differentiation factors. In combination with IL-7, SCF stimulates the proliferation of pre-B cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton. |
| Organism | Human (H. sapiens) |
| Unit quantity | 10 x 100 µg |
| Family | Other Growth Factors/Cytokines |
| Tag | Tag Free |
| Aliases |
DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF, WS2F |
| Gene Symbol | KITLG |
| Gene ID | 4254 |
| Accession Number | P21583 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 18.7 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity |
>95% as determined by SDS-PAGE |
| Formulation | Recombinant SCF was lyophilized from a 0.2 μm filtered solution in PBS pH 7.0. |
| Function | The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be ≤ 0.1 ng/mL |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Shipping Conditions | This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions. |
| Storage | Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100819 |
| How do I choose the right growth factor for my experiment? | |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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