Recombinant Mouse IL5

Cat. No.

Z200475

Unit

25 µg

Price

$320.00

Cat. No.	Z200475
Name	Recombinant Mouse IL5
Description	Interleukin-5 is a specific hematopoietic growth factor that is responsible for the growth and differentiation of eosinophils. IL-5 promotes the growth of immature hematopoietic progenitor cells BFU-E while it causes differentiation of CFU-E the proliferation of which is inhibited by IL-5. IL-5 strongly stimulates the proliferation, cell activation, and differentiation of eosinophils. B cells can be made responsive to IL-5 by treatment with suboptimal doses of IL-1. Interleukin-5 also promotes the generation of cytotoxic T cells from thymocytes. In thymocytes IL-5 induces the expression of high affinity IL-2 receptors. In contrast to human Interleukin-5, mouse IL-5 also acts on B cells. It induces the proliferation of pre-activated B cells and their differentiation. High affinity and low affinity receptors for IL-5 are expressed in all hematopoietic and lymphoid cells.The low affinity receptor, CD125, has a molecular mass of 50 kDa (p60). The introduction of p60 into these cells generates a high affinity IL-5 receptor. The second subunit of the IL-5 receptor is identical with the larger subunit of the receptor for the colony stimulating factor GM-CSF (CD131). This subunit is involved also in the generation of a high affinity receptor for IL-3 and is being referred to as common beta.
Organism	Mouse (M. musculus)
Unit quantity	25 µg
Family	Other Interleukins
Tag	Tag Free
Aliases	Il-5
Gene Symbol	IL5
Gene ID	16191
Accession Number	P04401
Source	Insect cells
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	13 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant Interleukin-5 was lyophilized from a 0.2 μm filtered PBS solution.
Function	The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤ 0.2 ng/mL, corresponding to a specific activity of ≥ 6.0 x 10^6 units/mg.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200475

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.