Retrovirus Packaging Combo Mix
| Cat. No. | E-510 | ||||||
| Name | Retrovirus Packaging Combo Mix | ||||||
| Unit | 75 µg | ||||||
| Description |
abm’s Retrovirus Packaging Plasmid Mix offers a high-efficiency solution for recombinant retrovirus production. This second generation system requires three plasmids for viral particle production: The ready-to-use Retrovirus Packaging Mix combines optimized Packaging and Envelope Plasmids for high viral titers and reliable gene delivery performance.
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| Storage Condition | -20°C or below. | ||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E-510 |
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Supporting Protocol
| Is it possible to propagate the packaging plasmids from this product mix? | |
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No, it is not possible to grow up the plasmids from the product.
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| What cell line should I use for retrovirus production? | |
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We recommend using HEK293T cell line due to its high transfection efficiency and virus yield.
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| What is the concentration of the Retrovirus Packaging Combo Mix? | |
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75 µg at a concentration of 0.5 µg/µl.
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| Can retrovirus infect non-dividing cells? | |
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No. Retrovirus require cell division for genome integration, unlike lentiviruses, which can infect both dividing and non-dividing cells.
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| Do you also offer retroviral transfer plasmids? | |
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Yes. We offer several retroviral transfer plasmids as well as Custom Recombinant Retrovirus Cloning and Packaging services.
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- Errington, Timothy M et al. Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology. (2021) eLife 10:e73430. Application: Retrovirus packaging
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